Isolation of Mycobacteria from Frozen Fish Destined for Human Consumption

Isolation of mycobacteria from frozen fish destined for human consumption.

Mycobacteria were isolated from defrost water and tissue of sole (Solea solea), hake (Merluccius merluccius), cod (Gadus morhua), ling (Genypterus blacodes), and monkfish (Lophius piscatorius) on Löwenstein-Jensen medium after incubation at different temperatures. Samples of frozen fish were obtained under sterile conditions inside a refrigeration chamber (-18 to -22 degrees C) in a wholesale m...

Characterisation of Aeromonas spp. isolated from frozen fish intended for human consumption in Mexico.

A total of 82 strains of presumptive Aeromonas spp. were identified biochemically and genetically (16S rDNA-RFLP). The strains were isolated from 250 samples of frozen fish (Tilapia, Oreochromis niloticus niloticus) purchased in local markets in Mexico City. In the present study, we detected the presence of several genes encoding for putative virulence factors and phenotypic activities that may...

Optimized Method for Isolation of Nontuberculous Mycobacteria from Hospital Aquatic Sources

Background: Aquatic ecosystems are an important source of nontuberculous mycobacteria (NTM) that can cause different diseases in human. Since culture of mycobacteria needs long-term incubation, fast-growing microorganisms and contaminants in the environment usually prevents the isolation of mycobacteria. Here, we compare different treatment protocols and describe a method that increases the rec...

Isolation of Mycobactins from Various Mycobacteria

Mycobactin S has been isolated from Mycobacterium smegmati8 and from Mycobacterium sp. Olitzky & Gershon, strain 2, and mycobactin H from M. thermore8i8tible; all three organisms were grown on synthetic media of low iron content. These two mycobactins are mixtures of compounds having the same nucleus but differing in their fatty side chains. The nucleus of mycobactin S has a chemical structure ...

Isolation of genomic DNA from mycobacteria.